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 | Acesso ao texto completo restrito à biblioteca da Embrapa Mandioca e Fruticultura. Para informações adicionais entre em contato com cnpmf.biblioteca@embrapa.br. |
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
25/08/2020 |
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Data da última atualização: |
25/08/2020 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
ARENA, G. D.; RAMOS-GONZALEZ, P. L.; FALK, B. W.; CASTEEL, C. L.; ASTUA, J. de F.; MACHADO, M. A. |
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Afiliação: |
GABRIELLA D. ARENA, Centro de Citricultura Sylvio Moreira; PEDRO LUIS RAMOS-GONZALEZ, Instituto Biológico; BRYCE W. FALK, University of California; CLARE L. CASTEEL, CASTEEL; JULIANA DE FREITAS ASTUA, CNPMF; MARCOS A. MACHADO, Centro de Citricultura Sylvio Moreira. |
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Título: |
Plant immune system activation upon Citrus Leprosis Virus c infection is mimicked by the ectopic expression of the P61 viral protein. |
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Ano de publicação: |
2020 |
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Fonte/Imprenta: |
Frontiers in Plant Science, August, 2020. |
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Idioma: |
Inglês |
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Conteúdo: |
Citrus leprosis virus C (CiLV-C, genus Cilevirus, family Kitaviridae) is an atypical virus that does not spread systemically in its plant hosts. Upon its inoculation by Brevipalpus mites, only localized lesions occur, and the infection remains limited to cells around mite feeding sites. Here, we aimed to gain insights into the putative causes of viral unfitness in plants by expanding the limited knowledge of the molecular mechanisms underlying plant/kitavirid interactions. Firstly, we quantified the CiLV-C viral RNAs during the infection in Arabidopsis thaliana plants using RT-qPCR and systematized it by defining three stages of distinguishing subgenomic and genomic RNA accumulation: i) 0–24 h after infestation, ii) 2–4 days after infestation (dai), and iii) 6–10 dai. Accordingly, the global plant response to CiLV-C infection was assessed by RNA-Seq at each period. Results indicated a progressive reprogramming of the plant transcriptome in parallel to the increasing viral loads. Gene ontology enrichment analysis revealed the induction of cell growth-related processes at the early stages of the infection and the triggering of the SA-mediated pathway, ROS burst and hypersensitive response (HR) at the presymptomatic stage. Conversely, infected plants downregulated JA/ET-mediated pathways and processes involved in the primary metabolism including photosynthesis. Marker genes of unfolded protein response were also induced, suggesting a contribution of the endoplasmic reticulum stress to the cell death caused by the viral infection. Finally, we transiently expressed CiLV-C proteins in Nicotiana benthamiana plants to undertake their roles in the elicited plant responses. Expression of the CiLV-C P61 protein consistently triggered ROS burst, upregulated SA- and HR-related genes, increased SA levels, reduced JA levels, and caused cell death. Mimicry of responses typically observed during CiLV-C–plant interaction indicates P61 as a putative viral effector causing the HR-like symptoms associated with the infection. Our data strengthen the hypothesis that symptoms of CiLV-C infection might be the outcome of a hypersensitive-like response during an incompatible interaction. Consequently, the locally restricted infection of CiLV-C, commonly observed across infections by kitavirids, supports the thesis that these viruses, likely arising from an ancestral arthropod-infecting virus, are unable to fully circumvent plant defenses. MenosCitrus leprosis virus C (CiLV-C, genus Cilevirus, family Kitaviridae) is an atypical virus that does not spread systemically in its plant hosts. Upon its inoculation by Brevipalpus mites, only localized lesions occur, and the infection remains limited to cells around mite feeding sites. Here, we aimed to gain insights into the putative causes of viral unfitness in plants by expanding the limited knowledge of the molecular mechanisms underlying plant/kitavirid interactions. Firstly, we quantified the CiLV-C viral RNAs during the infection in Arabidopsis thaliana plants using RT-qPCR and systematized it by defining three stages of distinguishing subgenomic and genomic RNA accumulation: i) 0–24 h after infestation, ii) 2–4 days after infestation (dai), and iii) 6–10 dai. Accordingly, the global plant response to CiLV-C infection was assessed by RNA-Seq at each period. Results indicated a progressive reprogramming of the plant transcriptome in parallel to the increasing viral loads. Gene ontology enrichment analysis revealed the induction of cell growth-related processes at the early stages of the infection and the triggering of the SA-mediated pathway, ROS burst and hypersensitive response (HR) at the presymptomatic stage. Conversely, infected plants downregulated JA/ET-mediated pathways and processes involved in the primary metabolism including photosynthesis. Marker genes of unfolded protein response were also induced, suggesting a contribution of the endoplasmic reticulum st... Mostrar Tudo |
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Thesagro: |
Fruta Cítrica. |
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Categoria do assunto: |
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Marc: |
LEADER 03074naa a2200193 a 4500
001 2124551
005 2020-08-25
008 2020 bl uuuu u00u1 u #d
100 1 $aARENA, G. D.
245 $aPlant immune system activation upon Citrus Leprosis Virus c infection is mimicked by the ectopic expression of the P61 viral protein.$h[electronic resource]
260 $c2020
520 $aCitrus leprosis virus C (CiLV-C, genus Cilevirus, family Kitaviridae) is an atypical virus that does not spread systemically in its plant hosts. Upon its inoculation by Brevipalpus mites, only localized lesions occur, and the infection remains limited to cells around mite feeding sites. Here, we aimed to gain insights into the putative causes of viral unfitness in plants by expanding the limited knowledge of the molecular mechanisms underlying plant/kitavirid interactions. Firstly, we quantified the CiLV-C viral RNAs during the infection in Arabidopsis thaliana plants using RT-qPCR and systematized it by defining three stages of distinguishing subgenomic and genomic RNA accumulation: i) 0–24 h after infestation, ii) 2–4 days after infestation (dai), and iii) 6–10 dai. Accordingly, the global plant response to CiLV-C infection was assessed by RNA-Seq at each period. Results indicated a progressive reprogramming of the plant transcriptome in parallel to the increasing viral loads. Gene ontology enrichment analysis revealed the induction of cell growth-related processes at the early stages of the infection and the triggering of the SA-mediated pathway, ROS burst and hypersensitive response (HR) at the presymptomatic stage. Conversely, infected plants downregulated JA/ET-mediated pathways and processes involved in the primary metabolism including photosynthesis. Marker genes of unfolded protein response were also induced, suggesting a contribution of the endoplasmic reticulum stress to the cell death caused by the viral infection. Finally, we transiently expressed CiLV-C proteins in Nicotiana benthamiana plants to undertake their roles in the elicited plant responses. Expression of the CiLV-C P61 protein consistently triggered ROS burst, upregulated SA- and HR-related genes, increased SA levels, reduced JA levels, and caused cell death. Mimicry of responses typically observed during CiLV-C–plant interaction indicates P61 as a putative viral effector causing the HR-like symptoms associated with the infection. Our data strengthen the hypothesis that symptoms of CiLV-C infection might be the outcome of a hypersensitive-like response during an incompatible interaction. Consequently, the locally restricted infection of CiLV-C, commonly observed across infections by kitavirids, supports the thesis that these viruses, likely arising from an ancestral arthropod-infecting virus, are unable to fully circumvent plant defenses.
650 $aFruta Cítrica
700 1 $aRAMOS-GONZALEZ, P. L.
700 1 $aFALK, B. W.
700 1 $aCASTEEL, C. L.
700 1 $aASTUA, J. de F.
700 1 $aMACHADO, M. A.
773 $tFrontiers in Plant Science, August, 2020.
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Embrapa Mandioca e Fruticultura (CNPMF) |
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| Acesso ao texto completo restrito à biblioteca da Embrapa Pecuária Sudeste. Para informações adicionais entre em contato com cppse.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Pecuária Sudeste. |
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Data corrente: |
29/09/2023 |
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Data da última atualização: |
28/11/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 1 |
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Autoria: |
ROMANELLO, N.; BARRETO, A. DO N.; SOUZA, M. A. P. DE; BALIEIRO, J. C. DE C.; BRANDÃO, F. Z.; TONATO, F.; BERNARDI, A. C. de C.; PEZZOPANE, J. R. M.; PORTUGAL, J. A. B.; GARCIA, A. R. |
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Afiliação: |
NARIAN ROMANELLO, University of São Paulo; ANDRÉA DO NASCIMENTO BARRETO, Federal University of Pará; MARCO ANTONIO PAULA DE SOUSA, Federal University of Pará; JÚLIO CESAR DE CARVALHO BALIEIRO, University of São Paulo; FELIPE ZANDONADI BRANDÃO, Fluminense Federal University; FELIPE TONATO, CPPSE; ALBERTO CARLOS DE CAMPOS BERNARDI, CPPSE; JOSE RICARDO MACEDO PEZZOPANE, CPPSE; JOSE ALBERTO BASTOS PORTUGAL, CPPSE; ALEXANDRE ROSSETTO GARCIA, CPPSE. |
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Título: |
Thermal comfort of Nelore (Bos indicus) and Canchim (Bos taurus x Bos indicus) bulls kept in an integrated crop-livestock-forestry system in a tropical climate. |
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Ano de publicação: |
2023 |
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Fonte/Imprenta: |
Agricultural Systems, v. 209, June 2023, 103687. |
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Páginas: |
12 p. |
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DOI: |
https://doi.org/10.1016/j.agsy.2023.103687 |
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Idioma: |
Inglês |
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Conteúdo: |
CONTEXT: Climate change presents challenges for livestock productivity and animal health. Thus, management strategies to mitigate the effects of the global temperature increase on livestock production have become progressively relevant. However, the use of integrated crop-livestock-forestry systems to optimize the beef cattle thermal balance has not been examined more deeply. OBJECTIVE: The study aimed to evaluate the microclimate in a non-shaded pasture system (NS) and in an integrated crop-livestock-forestry system (ICLF) and its influence on thermoregulatory and endocrine responses of zebu and composite bulls, during different climatic seasons. METHODS: The experiment was carried out in a tropical region, São Carlos-SP, Brazil (21o57'42"S, 47o50'28"W). Nelore (Bos indicus) and Canchim (5/8 Bos taurus x 3/8 Bos indicus) bulls were equally allocated in a non-shaded system (NS; n = 32) or in an integrated crop-livestock-forestry system (ICLF; n = 32). The animals were monthly evaluated and the data were analyzed using a GLM Model. Means were compared using Tukey test (P < 0.05). |
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Palavras-Chave: |
ILPF; Infrared thermography; Integrated systems; Sustainability. |
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Thesaurus NAL: |
Animal welfare; Thermoregulation; Tropical agriculture. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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Marc: |
LEADER 02170naa a2200337 a 4500
001 2156986
005 2023-11-28
008 2023 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1016/j.agsy.2023.103687$2DOI
100 1 $aROMANELLO, N.
245 $aThermal comfort of Nelore (Bos indicus) and Canchim (Bos taurus x Bos indicus) bulls kept in an integrated crop-livestock-forestry system in a tropical climate.$h[electronic resource]
260 $c2023
300 $a12 p.
520 $aCONTEXT: Climate change presents challenges for livestock productivity and animal health. Thus, management strategies to mitigate the effects of the global temperature increase on livestock production have become progressively relevant. However, the use of integrated crop-livestock-forestry systems to optimize the beef cattle thermal balance has not been examined more deeply. OBJECTIVE: The study aimed to evaluate the microclimate in a non-shaded pasture system (NS) and in an integrated crop-livestock-forestry system (ICLF) and its influence on thermoregulatory and endocrine responses of zebu and composite bulls, during different climatic seasons. METHODS: The experiment was carried out in a tropical region, São Carlos-SP, Brazil (21o57'42"S, 47o50'28"W). Nelore (Bos indicus) and Canchim (5/8 Bos taurus x 3/8 Bos indicus) bulls were equally allocated in a non-shaded system (NS; n = 32) or in an integrated crop-livestock-forestry system (ICLF; n = 32). The animals were monthly evaluated and the data were analyzed using a GLM Model. Means were compared using Tukey test (P < 0.05).
650 $aAnimal welfare
650 $aThermoregulation
650 $aTropical agriculture
653 $aILPF
653 $aInfrared thermography
653 $aIntegrated systems
653 $aSustainability
700 1 $aBARRETO, A. DO N.
700 1 $aSOUZA, M. A. P. DE
700 1 $aBALIEIRO, J. C. DE C.
700 1 $aBRANDÃO, F. Z.
700 1 $aTONATO, F.
700 1 $aBERNARDI, A. C. de C.
700 1 $aPEZZOPANE, J. R. M.
700 1 $aPORTUGAL, J. A. B.
700 1 $aGARCIA, A. R.
773 $tAgricultural Systems$gv. 209, June 2023, 103687.
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